| Expression pattern: |
UP |
| Associated gene: |
MDM2, p53, p21 |
| Associated microRNA: |
miR-1233-3p |
| Biological function: |
Promotes thyroid cancer cell proliferation and cell viability; decreases p53 protein level through increasing MDM2 expression via miR-1233-3p sponging. |
| Molecular mechanism: |
CircTP53 functions as a ceRNA by sponging miR-1233-3p, thereby increasing MDM2 expression and reducing p53 protein level. |
| Biological pathway or process: |
proliferation (promotes); p53 signaling (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
H
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; ISH (In Situ Hybridization); IHC (Immunohistochemistry); Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; MTT; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
CircTP53 is up-regulated in thyroid cancer tissues and mainly localizes in the cytoplasm of TPC-1 cells. It promotes thyroid cancer cell proliferation and viability by sponging miR-1233-3p, increasing MDM2 expression, and decreasing p53 protein signaling. |
| Confidence score: |
0.8566 |