| Expression pattern: |
UP |
| Associated gene: |
Cyclin B1, PCNA, Bax, cleaved caspase-3, Ki67 |
| Associated microRNA: |
miR-143-3p |
| Biological function: |
circTUBA1C promotes NSCLC cell proliferation and tumor growth, and inhibits cell apoptosis. |
| Molecular mechanism: |
circTUBA1C acts as a molecular sponge for miR-143-3p and negatively regulates miR-143-3p, thereby promoting proliferation, inhibiting apoptosis, and accelerating tumor growth in NSCLC. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; Luciferase Reporter Assay; Bioinformatics Analysis; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
- |
| Description: |
circTUBA1C is up-regulated in NSCLC tissues and cell lines. It promotes NSCLC cell proliferation and xenograft tumor growth while inhibiting apoptosis, at least partly by sponging and negatively regulating miR-143-3p. Knockdown of circTUBA1C suppresses Cyclin B1 and PCNA and increases Bax and cleaved caspase-3, effects that can be rescued by miR-143-3p inhibition. |
| Confidence score: |
0.7126 |