circRNA basic information
circBase ID: hsa_circ_0006404
Name: hsa_circ_FOXO3
Synonym: CircFOXO3
Host Gene: FOXO3
Genomic location(hg19): chr6:108984657-108986092:+
Genomic location(hg38): chr6:108663454-108664889:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0004950
MONDO name: gastric carcinoma
Disease details: gastric carcinoma
Disease DO ID:
 10538, 5517
Disease MeSH ID:
 -
Disease NCIt ID:
 C4911
Disease ICD11 ID:
 1503707183
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

GC tumors
Cell lines:

GES1; AGS; MKN45; SNU5; MKN74; MGC803; NCI-N87; 293T
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: USP44 mRNA, USP44, AGO2
Associated microRNA: miR-143-3p
Biological function: Promotes gastric carcinoma cell proliferation, migration, and in vivo tumor growth.
Molecular mechanism: CircFOXO3 functions as a molecular sponge for miR-143-3p and promotes gastric carcinoma progression by upregulating USP44 through the miR-143-3p-USP44 axis.
Biological pathway or process:

proliferation (promotes); migration (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Bioinformatics Analysis; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; RNA Pull-Down; Transfection; CCK8; Transwell Assay; Western Blot; In Vivo Animal Model
Clinical significance:

-
Description:

CircFOXO3 is reported to be upregulated in gastric carcinoma models and to promote malignant phenotypes, including proliferation, migration, and xenograft tumor growth. Mechanistically, circFOXO3 acts as a sponge/scaffold for miR-143-3p, relieving miR-143-3p-mediated repression of USP44 and thereby enhancing the miR-143-3p-USP44 axis in GC progression.
Confidence score:

0.6562
Other information
Title:

CircFOXO3 functions as a molecular sponge for miR-143-3p to promote the progression of gastric carcinoma via upregulating USP44.

Journal: Gene
Published: 2020
PubMed ID: 32445925
Study type:

biological research
Data availability: The data sets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Code availability: -