| Expression pattern: |
UP |
| Associated gene: |
CDK2, Ago2 |
| Associated microRNA: |
miR-1296 |
| Biological function: |
promotes cervical cancer cell proliferation and cell cycle progression; suppresses apoptosis |
| Molecular mechanism: |
ceRNA mechanism: hsa_circ_0000520 sponges miR-1296 (Ago2-associated) to relieve repression of CDK2 |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; EdU Staining; CCK8; Colony Formation Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model |
| Clinical significance: |
potential biomarker of prognosis and a therapeutic target |
| Description: |
hsa_circ_0000520 is up-regulated in cervical cancer tissues and cell lines and mainly localized in the cytoplasm. It promotes proliferation and cell-cycle progression and inhibits apoptosis by acting as an Ago2-associated sponge of miR-1296, thereby increasing CDK2 expression; knockdown suppresses xenograft tumor growth. |
| Confidence score: |
0.7396 |