circRNA basic information
circBase ID: hsa_circ_0008285
Name: hsa_circ_CDYL
Synonym: -
Host Gene: CDYL
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer
Disease DO ID:
5672, 9256
Disease MeSH ID:
-
Disease NCIt ID:
C4978
Disease ICD11 ID:
-
Disease OMIM ID:
114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

CRC tissues; matched noncancerous colorectal mucosa; non-cancerous tissues

Cell lines:

SW480; RKO; HCT8; SW620; HCT116; DLD1; FHC; 293T

In vivo animal model:

-

circRNA-disease information
Expression pattern:
DN
Associated gene: PTEN
Associated microRNA: miR-382-5p
Biological function: Tumor suppressor role in CRC; inhibits CRC cell proliferation and migration.
Molecular mechanism: circ_0008285 sponges miR-382-5p to elevate PTEN expression and suppresses the PI3K/AKT pathway.
Biological pathway or process:

PI3K/AKT (inhibits); proliferation (inhibits); migration (inhibits); ceRNA regulation (other)

Detected method:
Q
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Transwell Assay; RNA-seq; Bioinformatics Analysis; Luciferase Reporter Assay; Western Blot

Clinical significance:

Decreased circ_0008285 expression was correlated with larger tumor size, more lymphatic metastasis, and higher TNM stage; circ_0008285 may be a potential therapeutic target for CRC treatment.

Description:

hsa_circ_0008285/circ_0008285 is downregulated in CRC tissues and cell lines and acts as a tumor-suppressive circRNA. It inhibits CRC cell proliferation and migration by sponging miR-382-5p, upregulating PTEN, and suppressing PI3K/AKT signaling; lower expression correlates with larger tumor size, lymphatic metastasis, and advanced TNM stage.

Confidence score:

0.802

Other information
Title:

Circular RNA hsa_circ_0008285 inhibits colorectal cancer cell proliferation and migration via the miR-382-5p/PTEN axis.

Journal: Biochemical and biophysical research communications
Published: 2020
PubMed ID: 32423803
Study type:

combined biological and clinical study

Data availability: NCBI Sequence Read Archive (SRA) database: https://www.ncbi.nlm.nih.gov/sra/PRJNA604411; Supplementary Table 1; Supplementary Table 2; Supplementary data: https://doi.org/10.1016/j.bbrc.2020.03.165
Code availability: -