circRNA basic information
circBase ID: hsa_circ_0081723
Name: hsa_circ_POLR2J2
Synonym: circ_0081723
Host Gene: POlR2J2
Genomic location(hg19): chr7:102307536-102309451:-
Genomic location(hg38): chr7:102667089-102669004:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0002974
MONDO name: cervical cancer
Disease details: cervical cancer
Disease DO ID:
4362
Disease MeSH ID:
-
Disease NCIt ID:
C9311
Disease ICD11 ID:
1256072522
Disease OMIM ID:
603956
Species: Human
Species details: Homo sapiens
Tissue specimen:

CC tumor tissue samples; adjacent no-tumor tissues; xenograft tumor tissues

Cell lines:

SiHa; Caski; Ect1/E6E7

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: CREBRF, Ago2
Associated microRNA: miR-545-3p
Biological function: Promotes cervical cancer cell proliferation, migration, invasion, EMT-associated malignant behaviors and xenograft tumor growth, while inhibiting apoptosis.
Molecular mechanism: circ_0081723 functions as a ceRNA/molecular sponge for miR-545-3p, thereby regulating CREBRF expression and promoting cervical cancer progression.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); EMT (promotes)

Detected method:
Q
Validation methods:

RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Bioinformatics Analysis

Clinical significance:

circ_0081723 might be a biomarker and a possible circRNA-targeted therapeutic target for cervical cancer.

Description:

circ_0081723 is up-regulated in cervical cancer tissues and cell lines and acts as an oncogenic circRNA. It promotes CC cell proliferation, migration, invasion and xenograft tumor growth while suppressing apoptosis, mainly through sponging miR-545-3p to regulate CREBRF. The study suggests circ_0081723 may serve as a biomarker and therapeutic target for cervical cancer.

Confidence score:

0.8175

Other information
Title:

Circ_0081723 enhances cervical cancer progression and modulates CREBRF via sponging miR-545-3p.

Journal: Naunyn-Schmiedeberg's archives of pharmacology
Published: 2024
PubMed ID: 38850307
Study type:

combined biological and clinical study

Data availability: No datasets were generated or analysed during the current study; supplementary material available at https:// doi. org/ 10. 1007/ s00210-024-03175-8
Code availability: -