| Expression pattern: |
UP |
| Associated gene: |
TGF-beta1 |
| Associated microRNA: |
miR-30a |
| Biological function: |
Exacerbates renal tubulointerstitial fibrosis and promotes profibrotic gene/protein production (TGF-beta1, FN, COL1) in vivo and in HK-2 cells. |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-30a, relieving repression of TGF-beta1 and forming a feed-forward loop among circHIPK3/miR-30a/TGF-beta1. |
| Biological pathway or process: |
fibrosis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
|
| Validation methods: |
RT-qPCR; FISH / smFISH; Transfection; Western Blot; IF (Immunofluorescence); In Vivo Animal Model; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
circHIPK3 may be a novel therapeutic target for slowing CKD progression / renal fibrosis. |
| Description: |
circHIPK3 is up-regulated in folic acid-induced renal tubulointerstitial fibrosis and in human cTIN fibrotic kidney tissue. It localizes mainly to the cytoplasm and exacerbates fibrosis by sponging miR-30a, thereby increasing TGF-beta1 and downstream profibrotic factors (FN, COL1) in a feed-forward loop. |
| Confidence score: |
0.6046 |