| Expression pattern: |
UP |
| Associated gene: |
UBE2T, CD44, PCNA, Bax, Slug, SOX2 |
| Associated microRNA: |
miR-1322 |
| Biological function: |
Circ_0000291 promotes HCC cell proliferation, migration, invasion, stemness and tumor growth, and inhibits apoptosis. |
| Molecular mechanism: |
Circ_0000291 acts as a miR-1322 sponge to positively regulate UBE2T expression, thereby promoting HCC progression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); stemness (promotes); apoptosis (inhibits); metastasis (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; Cohort Study; Survival Analysis; Bioinformatics Analysis; Transfection; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; Luciferase Reporter Assay; RNA Pull-Down; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
High circ_0000291 expression is associated with lower survival rate and may be a novel prognostic marker and potential biomarker/target for HCC patients. |
| Description: |
Circ_0000291 is up-regulated in HCC tissues and cell lines and high expression predicts poor survival. Functionally, circ_0000291 promotes proliferation, migration, invasion, stemness and in vivo tumor growth while suppressing apoptosis. Mechanistically, it acts as a sponge for miR-1322 to up-regulate UBE2T, forming a circ_0000291/miR-1322/UBE2T axis that supports HCC progression. |
| Confidence score: |
0.8281 |