| Expression pattern: |
DN |
| Associated gene: |
MBD2, FGFR4, NFYA |
| Associated microRNA: |
miR-520c-3p |
| Biological function: |
Suppresses HCC recurrence and inhibits HCC cell proliferation, colony formation, migration, and tumor formation; modulates lenvatinib response. |
| Molecular mechanism: |
Transcriptionally repressed by NFYA; acts as a miRNA sponge for miR-520c-3p to regulate MBD2 and downstream FGFR4. |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); cell cycle (inhibits); apoptosis (promotes); ceRNA regulation (other); other pathway/process (inhibits); drug resistance (other) |
| Detected method: |
Q
S
|
| Validation methods: |
circRNA-seq; RNA-seq; Bioinformatics Analysis; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RT-qPCR; Clinical Sample Validation; Cohort Study; Survival Analysis; circRIP; RNA Pull-Down; Luciferase Reporter Assay; ChIP / ChIP-seq; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Cell Cycle Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; Annexin V/PI Flow Cytometry |
| Clinical significance: |
Lower circKCNN2 in tumors is associated with recurrence; elevated circKCNN2 in tumors predicts favorable overall survival and recurrence-free survival and is associated with decreased risk of HCC recurrence. |
| Description: |
circKCNN2 is downregulated in HCC and acts as a tumor-suppressive circRNA associated with lower recurrence risk and better survival. Mechanistically, it is repressed by NFYA and suppresses HCC progression by sponging miR-520c-3p to modulate MBD2 and downstream FGFR4, influencing lenvatinib response. |
| Confidence score: |
0.8913 |