| Expression pattern: |
UP |
| Associated gene: |
SMAD2, TGF-beta, WNT1 |
| Associated microRNA: |
miR-1276 |
| Biological function: |
promotes bladder cancer cell proliferation, migration, cell cycle progression, tumor growth and lung metastasis |
| Molecular mechanism: |
ceRNA mechanism: circ_0002623 sponges miR-1276 to upregulate SMAD2, affecting TGF-beta and WNT1 expression |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); cell cycle (promotes); metastasis (promotes); TGF-beta/SMAD (promotes); Wnt/beta-catenin (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; Sanger Sequencing; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; BrdU; Transwell Assay; Cell Cycle Assay; Luciferase Reporter Assay; Western Blot; In Vivo Animal Model; H&E Staining; Survival Analysis |
| Clinical significance: |
high circ_0002623 expression was positively correlated with lymph node metastasis and associated with shorter overall survival of BCa patients |
| Description: |
circ_0002623 is upregulated in bladder cancer and acts as an oncogenic circRNA. It mainly localizes to the cytoplasm and promotes proliferation, migration, cell-cycle progression and in vivo tumor growth/metastasis by sponging miR-1276 to increase SMAD2, thereby enhancing TGF-beta and Wnt-related signaling (e.g., WNT1). High circ_0002623 expression correlates with lymph node metastasis and poorer overall survival. |
| Confidence score: |
0.84 |