| Expression pattern: |
UP |
| Associated gene: |
BCL11A |
| Associated microRNA: |
miR-4753, miR-6809 |
| Biological function: |
promotes TNBC cell proliferation and tumor growth; inhibits apoptosis |
| Molecular mechanism: |
circEPSTI1 acts as a miRNA sponge for miR-4753 and miR-6809 to regulate BCL11A (ceRNA mechanism) |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
H
M
|
| Validation methods: |
Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Luciferase Reporter Assay; Western Blot; ISH (In Situ Hybridization); IHC (Immunohistochemistry); In Vivo Animal Model; H&E Staining; Clinical Sample Validation; Cohort Study; Survival Analysis |
| Clinical significance: |
High levels of circEPSTI1 correlate with reduced survival in TNBC patients; independent prognostic marker for survival |
| Description: |
circEPSTI1 is up-regulated in TNBC tissues/cell lines and promotes proliferation and tumor growth while inhibiting apoptosis. Mechanistically, it acts as a ceRNA by sponging miR-4753 and miR-6809 to increase BCL11A expression. High circEPSTI1 expression is associated with worse DFS/OS and serves as an independent prognostic marker in TNBC. |
| Confidence score: |
0.8601 |