| Expression pattern: |
UP |
| Associated gene: |
YY1, PCNA, HK2, LDHA, ASCT2, GLS1 |
| Associated microRNA: |
miR-330-5p |
| Biological function: |
promotes proliferation, glycolysis, and glutamine catabolism/decomposition of NSCLC cells; promotes xenografted tumor growth |
| Molecular mechanism: |
circ_0000517 sponges miR-330-5p to regulate YY1, affecting proliferation, glycolysis and glutamine decomposition in NSCLC |
| Biological pathway or process: |
proliferation (promotes); glycolysis (promotes); glutaminolysis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Clinical Sample Validation; Survival Analysis; Luciferase Reporter Assay; RNA Pull-Down; Transfection; CCK8; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Bioinformatics Analysis |
| Clinical significance: |
Overexpression of circ_0000517 was negatively correlated with the prognosis of NSCLC patients; associated with tumor size, lymph node metastasis, and tumor differentiation |
| Description: |
circ_0000517 is up-regulated in NSCLC tissues and cell lines and its high expression is associated with worse overall survival. Functionally, circ_0000517 promotes NSCLC proliferation, glycolysis and glutamine catabolism and supports xenograft growth by acting as a ceRNA for miR-330-5p to increase YY1. |
| Confidence score: |
0.7942 |