| Expression pattern: |
DN |
| Associated gene: |
SMAD4 |
| Associated microRNA: |
miR-671-5p |
| Biological function: |
Exosomal circ_PTPRA induces CRC cell cycle arrest, inhibits CRC cell proliferation and tumor growth, promotes radiosensitivity, decreases colony formation, and increases apoptosis after radiation. |
| Molecular mechanism: |
circ_PTPRA functions as a ceRNA/miRNA sponge for miR-671-5p, thereby increasing SMAD4 expression and inhibiting CRC growth and radioresistance. |
| Biological pathway or process: |
ceRNA regulation (other); proliferation (inhibits); cell cycle (inhibits); apoptosis (promotes); radioresistance (inhibits) |
| Detected method: |
Q
S
|
| Validation methods: |
circRNA-seq; RT-qPCR; RNase R Treatment; Clinical Sample Validation; Bioinformatics Analysis; Transfection; MTT; Cell Cycle Assay; Colony Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; Luciferase Reporter Assay; In Vivo Animal Model |
| Clinical significance: |
Exosomal circ_PTPRA might be used as a potential predicted and therapeutic target or promising biomarker for CRC diagnosis, treatment and prognosis. |
| Description: |
circ_PTPRA is downregulated in serum-derived exosomes from CRC patients and in CRC cell lines. Exosomal circ_PTPRA suppresses CRC cell proliferation and tumor growth and enhances radiosensitivity by sponging miR-671-5p to increase SMAD4 expression. The study suggests exosomal circ_PTPRA may serve as a CRC biomarker and therapeutic target. |
| Confidence score: |
0.7595 |