| Expression pattern: |
UP |
| Associated gene: |
ACVR1C/ALK7, FZD3, IL6ST/GP130, SKIL/SNON, SMAD2, WNT5A |
| Associated microRNA: |
miR-140-3p, miR-382, miR-579 |
| Biological function: |
Modulates CSC stemness properties in CRC spheroid cells by sponging tumor suppressor miRNAs, leading to increased expression of stemness-related mRNA targets. |
| Molecular mechanism: |
ceRNA mechanism (circRNA sponging of miRNAs) regulating mRNA targets involved in pluripotency-related signaling pathways (GP130/Stat, Activin/Nodal, TGF-beta/SMAD, Wnt/beta-catenin). |
| Biological pathway or process: |
stemness (promotes); ceRNA regulation (promotes); Wnt/beta-catenin (promotes); TGF-beta/SMAD (promotes); JAK/STAT (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
circRNA-seq; RNase R Treatment; divergent primers PCR; RT-qPCR; Bioinformatics Analysis; Luciferase Reporter Assay |
| Clinical significance: |
May be included in a circRNA-miRNA-mRNA panel as a precision biomarker for diagnosis and prognosis of colorectal cancer progression. |
| Description: |
hsa_circ_0082096 is up-regulated in CSC-enriched CRC spheroid cells and sponges core miRNAs (including miR-140-3p, miR-382 and miR-579), leading to derepression of multiple mRNA targets (e.g., IL6ST/GP130, SMAD2, WNT5A) and activation of pluripotency-associated signaling that supports CSC stemness. |
| Confidence score: |
0.5719 |