| Expression pattern: |
UP |
| Associated gene: |
NOTCH1 |
| Associated microRNA: |
miR-34a-5p |
| Biological function: |
promotes glioma progression by enhancing proliferation and migration and inhibiting apoptosis |
| Molecular mechanism: |
ceRNA sponge of miR-34a-5p leading to upregulation of NOTCH1 and activation of the Notch signaling pathway |
| Biological pathway or process: |
Notch (promotes); proliferation (promotes); migration (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; Bioinformatics Analysis; RT-qPCR; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Wound Healing Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; Clinical Sample Validation |
| Clinical significance: |
- |
| Description: |
circNFIX is up-regulated in glioma tissues/cell lines and promotes glioma growth and motility. Mechanistically, circNFIX sponges miR-34a-5p to de-repress NOTCH1, activating Notch signaling and thereby enhancing proliferation/migration while reducing apoptosis; knockdown of circNFIX suppresses xenograft tumor growth. |
| Confidence score: |
0.73 |