| Expression pattern: |
UP |
| Associated gene: |
RBM3, ERK |
| Associated microRNA: |
- |
| Biological function: |
promotes HCC cell proliferation and cell cycle progression; increases xenografted tumor growth |
| Molecular mechanism: |
RBM3 binds SCD mRNA 3’UTR and promotes SCD-circRNA 2 biogenesis; SCD-circRNA 2 mediates RBM3-driven ERK phosphorylation to promote proliferation |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); ERK (promotes) |
| Detected method: |
Q
B
S
|
| Validation methods: |
RNA-seq; RNase R Treatment; RT-qPCR; Northern Blot; divergent primers PCR; Sanger Sequencing; Clinical Sample Validation; Cohort Study; Survival Analysis; ROC Analysis; RIP (RNA Immunoprecipitation); Transfection; CCK8; EdU Staining; Cell Cycle Assay; Western Blot; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
High SCD-circRNA 2 expression levels exhibited both worse recurrence-free survival (RFS) and overall survival (OS) and was an independent predictor for reduced RFS and OS in HCC patients |
| Description: |
SCD-circRNA 2 is up-regulated in HCC tissues and its high expression predicts worse RFS/OS. Functionally, it is required for RBM3-driven proliferation and cell-cycle progression, and it promotes ERK phosphorylation downstream of RBM3. |
| Confidence score: |
0.8618 |