| Expression pattern: |
DN |
| Associated gene: |
PC |
| Associated microRNA: |
- |
| Biological function: |
Suppresses breast cancer proliferation, migration, invasion, tumor growth and lung metastasis; promotes ROS accumulation and apoptosis; inhibits PC activity, aspartate synthesis and NADPH production. |
| Molecular mechanism: |
SF3B4-QKI splicing complex promotes FNDC3B back-splicing to generate Circ-FNDC3B. Circ-FNDC3B binds the biotin carboxylase domain of PC and inhibits PC activity, reducing aspartate synthesis and the citrate-pyruvate cycle, decreasing NADPH production, increasing ROS-mediated oxidative damage, and promoting apoptosis. |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); apoptosis (promotes); mitochondrial function (other); other pathway/process (inhibits) |
| Detected method: |
Q
S
|
| Validation methods: |
circRNA-seq; RT-qPCR; Clinical Sample Validation; ROC Analysis; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; EdU Staining; Transwell Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Co-IP; IF (Immunofluorescence); Flow Cytometry（Non-apoptosis/cycle）; Annexin V/PI Flow Cytometry; Western Blot; Bioinformatics Analysis; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry) |
| Clinical significance: |
Circ-FNDC3B expression is associated with tumor diameter, clinical stage and lymph node metastasis, and ROC analysis suggests it may serve as a biomarker for differentiating breast cancer from nontumorous tissue and predicting lymph node metastasis. |
| Description: |
Circ-FNDC3B is a human FNDC3B-derived circRNA that is down-regulated in breast cancer, especially in cases with lymph node metastasis. It suppresses breast cancer progression by binding PC and inhibiting its activity, thereby reducing aspartate and NADPH production, increasing ROS accumulation, promoting apoptosis, and inhibiting proliferation, invasion and metastasis. Its expression is clinically associated with tumor diameter, clinical stage and lymph node metastasis, suggesting biomarker potential. |
| Confidence score: |
0.8769 |