| Expression pattern: |
UP |
| Associated gene: |
SOCS3 |
| Associated microRNA: |
miR-203 |
| Biological function: |
Promotes BOO-associated bladder hypertrophy and fibrogenesis and contributes to hypoxia-induced fibrotic phenotypic changes in BSMCs; its knockdown alleviates fibrosis-related gene expression changes, improves cell viability, and suppresses hypoxia-induced apoptosis. |
| Molecular mechanism: |
Acts as a ceRNA by sponging miR-203, thereby relieving miR-203-mediated repression of SOCS3 (circPVT1-miR-203-SOCS3 axis). |
| Biological pathway or process: |
fibrosis (promotes); proliferation (other); apoptosis (other); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; Western Blot; CCK8; Annexin V/PI Flow Cytometry; H&E Staining; In Vivo Animal Model |
| Clinical significance: |
circPVT1 is upregulated in bladder biopsies from BOO patients versus healthy controls. |
| Description: |
circPVT1 is upregulated in BOO-associated bladder remodeling and in hypoxia-induced fibrotic BSMCs. It promotes bladder hypertrophy/fibrosis by sponging miR-203, thereby increasing SOCS3 expression; circPVT1 knockdown ameliorates fibrotic markers and cellular injury phenotypes. |
| Confidence score: |
0.6829 |