| Expression pattern: |
UP |
| Associated gene: |
PDPK1 |
| Associated microRNA: |
miR-33a-5p |
| Biological function: |
promotes proliferation; promotes clonogenesis; promotes EMT; inhibits apoptosis |
| Molecular mechanism: |
circANXA2 acts as a miRNA sponge for miR-33a-5p to upregulate the downstream target PDPK1 (ceRNA mechanism). |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; FISH / smFISH; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; TUNEL; Western Blot; IHC (Immunohistochemistry); Bioinformatics Analysis; In Vivo Animal Model; Clinical Sample Validation |
| Clinical significance: |
circANXA2 is related to the prognosis of lung cancer patients. |
| Description: |
circANXA2 is upregulated in lung adenocarcinoma tissues and lung cancer cell lines and is mainly localized in the cytoplasm. It promotes malignant phenotypes (proliferation, clonogenesis and EMT) and inhibits apoptosis by sponging miR-33a-5p, thereby increasing PDPK1 expression. |
| Confidence score: |
0.7369 |