| Expression pattern: |
UP |
| Associated gene: |
ARL4C, IRF3, AGO2 |
| Associated microRNA: |
miR-1306-5p |
| Biological function: |
Promotes PDAC cell proliferation, migration, EMT and tumor growth; inhibits apoptosis. |
| Molecular mechanism: |
circUHRF1 acts as a miRNA sponge for miR-1306-5p to regulate ARL4C expression; circUHRF1 expression is transcriptionally regulated by IRF3. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); migration (promotes); EMT (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Bioinformatics Analysis; In Vivo Animal Model |
| Clinical significance: |
circUHRF1 is one promising diagnosis or therapeutic target for PDAC management. |
| Description: |
circUHRF1 is up-regulated in PDAC tissues and cell lines and mainly localizes in the cytoplasm. It promotes PDAC proliferation, migration, EMT and tumor growth while inhibiting apoptosis by sponging miR-1306-5p to upregulate ARL4C. Its expression is positively regulated by the transcription factor IRF3. |
| Confidence score: |
0.8279 |