| Expression pattern: |
UP |
| Associated gene: |
VIM, CDH1, Twist1 |
| Associated microRNA: |
miR-1246 |
| Biological function: |
Promotes EMT/mesenchymal phenotype, migration and invasion; inhibits apoptosis; promotes proliferation; associated with relapse risk in resected NSCLC. |
| Molecular mechanism: |
Likely ceRNA mechanism: circ-10720 modulates VIM via miR-1246 in NSCLC cells, supported by increased miR-1246 upon circ-10720 silencing and reduced VIM protein. |
| Biological pathway or process: |
EMT (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); proliferation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Survival Analysis; Transfection; Western Blot; IF (Immunofluorescence); Wound Healing Assay; Transwell Assay; MTT |
| Clinical significance: |
High circ-10720 expression is an independent prognostic marker for shorter time to relapse (TTR) in resected NSCLC patients not receiving adjuvant treatment. |
| Description: |
circ-10720 (hsa_circ_0018189, host gene CUL2) is upregulated in NSCLC tumor tissue and promotes EMT-associated phenotypes. Its knockdown reduces VIM protein, suppresses migration/invasion, increases apoptosis, and decreases proliferation; high tumor expression predicts shorter time to relapse in non-adjuvantly treated resected patients, with evidence consistent with a miR-1246-mediated ceRNA mechanism. |
| Confidence score: |
0.6211 |