| Expression pattern: |
UP |
| Associated gene: |
ELK1 |
| Associated microRNA: |
miR-326, miR-330-5p |
| Biological function: |
promotes proliferation; promotes migration; inhibits apoptosis |
| Molecular mechanism: |
ceRNA mechanism: circRNA_000166 targets miR-326 and miR-330-5p to regulate ELK1. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); apoptosis (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; Clinical Sample Validation; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circRNA_000166 expression was associated with shorter postoperative PFS and OS, and positively correlated with tumour size, TNM stage, histological grade, and lymph node metastasis. |
| Description: |
circRNA_000166 (circRPPH1) is up-regulated in breast cancer tissues and cell lines and acts as an oncogenic circRNA. It sponges miR-326 and miR-330-5p, thereby relieving repression of ELK1 and promoting proliferation/migration while inhibiting apoptosis; high expression is associated with worse postoperative survival (PFS/OS). |
| Confidence score: |
0.8423 |