circRNA basic information
circBase ID: hsa_circ_0081534
Name: hsa_circ_EPHB4
Synonym: circ_0081534
Host Gene: EPHB4
Genomic location(hg19): chr7:100417178-100417918:-
Genomic location(hg38): chr7:100819556-100820296:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0015459
MONDO name: nasopharyngeal carcinoma
Disease details: nasopharyngeal carcinoma
Disease DO ID:
 9261
Disease MeSH ID:
 D00007727; D000077274
Disease NCIt ID:
 C3871
Disease ICD11 ID:
 1883313543
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

NPC tissue specimens; normal nasopharyngeal tissues; xenograft tumor tissues
Cell lines:

5-8F; HNE-3; 6-10B; C666-1; NP69
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: FMNL3, Ago2, EPHB4
Associated microRNA: miR-874-3p
Biological function: Promotes NPC cell proliferation, migration, invasion, EMT, tumor growth, and inhibits apoptosis.
Molecular mechanism: circ_0081534 acts as a ceRNA/miRNA sponge for miR-874-3p, thereby upregulating FMNL3 expression to facilitate NPC progression.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); EMT (promotes); metastasis (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Bioinformatics Analysis; Transfection; MTT; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); In Vivo Animal Model; IHC (Immunohistochemistry)
Clinical significance:

circ_0081534 expression was positively related to tumor size, tumor stage, and lymph node metastasis of NPC patients; potential target for NPC treatment.
Description:

hsa_circ_0081534/circ_0081534 is upregulated in nasopharyngeal carcinoma tissues and cell lines and is associated with larger tumor size, advanced tumor stage, and lymph node metastasis. Functionally, it promotes NPC proliferation, migration, invasion, EMT, and tumor growth while suppressing apoptosis. Mechanistically, cytoplasmic circ_0081534 sponges miR-874-3p to increase FMNL3 expression, forming a circ_0081534/miR-874-3p/FMNL3 regulatory axis.
Confidence score:

0.8175
Other information
Title:

Hsa_circ_0081534 facilitates malignant phenotypes by sequestering miR-874-3p and upregulating FMNL3 in nasopharyngeal carcinoma.

Journal: Auris, nasus, larynx
Published: 2022
PubMed ID: 35428519
Study type:

combined biological and clinical study
Data availability: supporting data is not available
Code availability: -