| Expression pattern: |
UP |
| Associated gene: |
Ago2, JAK2, STAT3 |
| Associated microRNA: |
miR-486-3p, miR-637 |
| Biological function: |
promotes OSCC cell growth, migration, invasion and angiogenesis; inhibits apoptosis; promotes tumor growth in vivo |
| Molecular mechanism: |
acts as a miRNA sponge for miR-486-3p and miR-637, thereby activating the JAK2/STAT3 pathway |
| Biological pathway or process: |
JAK/STAT (promotes); proliferation (promotes); migration (promotes); invasion (promotes); angiogenesis (promotes); apoptosis (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Sanger Sequencing; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Tube Formation Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation |
| Clinical significance: |
might be a potential biomarker and therapeutic target for OSCC |
| Description: |
circ_0005320 is up-regulated in OSCC tissues and cell lines and functions as an oncogenic circRNA. It sponges miR-486-3p and miR-637, thereby promoting malignant phenotypes and activating the JAK2/STAT3 signaling pathway; its knockdown suppresses tumor growth in a nude mouse xenograft model. |
| Confidence score: |
0.8327 |