| Expression pattern: |
UP |
| Associated gene: |
PTP1B |
| Associated microRNA: |
hsa-miR-141-3p, hsa-miR-185-5p, hsa-miR-200a-3p, miR-338-3p |
| Biological function: |
promotes proliferation, migration and invasion/metastasis of osteosarcoma cells |
| Molecular mechanism: |
Acts as a ceRNA to sponge and inhibit miR-338-3p, thereby increasing PTP1B expression |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Transfection; Luciferase Reporter Assay; CCK8; Transwell Assay; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
circCCDC66 was overexpressed in tumor cells compared to normal tissues and had potential application value as a marker for screening and monitoring OS. |
| Description: |
circCCDC66 is up-regulated in osteosarcoma tissues and cell lines and promotes malignant phenotypes (proliferation, migration, invasion). Mechanistically, it acts mainly in the cytoplasm as a ceRNA sponging miR-338-3p to upregulate PTP1B. |
| Confidence score: |
0.7291 |