circRNA basic information
circBase ID: hsa_circ_0006990
Name: hsa_circ_VAPA
Synonym: -
Host Gene: VAPA
Genomic location(hg19): chr18:9931806-9937063:+
Genomic location(hg38): chr18:9931809-9937066:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer / CRC
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

THP-1; HCT116; LoVo
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: MUC13, E2F1
Associated microRNA: miR-132-3p, miR-342-3p
Biological function: Promotes CRC tumorigenesis; overexpression reverses quercetin-induced apoptosis and restores proliferation, migration and invasion.
Molecular mechanism: Quercetin suppresses CRC progression via a ceRNA mechanism in which hsa_circ_0006990 sponges miR-132-3p and miR-342-3p to regulate MUC13 and E2F1.
Biological pathway or process:

proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); ceRNA regulation (other)
Detected method:
Q
Validation methods:

Bioinformatics Analysis; RT-qPCR; Luciferase Reporter Assay; Transfection; Colony Formation Assay; TUNEL; Wound Healing Assay; Transwell Assay; Western Blot
Clinical significance:

-
Description:

In CRC cells (HCT116 and LoVo) under M2-TAM co-culture conditions, hsa_circ_0006990 is upregulated and promotes tumorigenic behaviors. Quercetin inhibits CRC proliferation, migration and invasion and promotes apoptosis partly by downregulating hsa_circ_0006990. Mechanistically, hsa_circ_0006990 sponges miR-132-3p and miR-342-3p to regulate MUC13 and E2F1, forming ceRNA axes involved in quercetin-mediated CRC suppression.
Confidence score:

0.5627
Other information
Title:

Quercetin Inhibits Tumorigenesis of Colorectal Cancer Through Downregulation of hsa_circ_0006990.

Journal: Frontiers in pharmacology
Published: 2022
PubMed ID: 35662705
Study type:

biological research
Data availability: https://www.frontiersin.org/articles/10.3389/fphar.2022.874696/full#supplementary-material
Code availability: -