| Expression pattern: |
UP |
| Associated gene: |
HMGB3, AGO2 |
| Associated microRNA: |
miR-195-5p |
| Biological function: |
Promotes NSCLC cell proliferation, migration, invasion, glycolysis and tumor growth; inhibits apoptosis and G0/G1 phase arrest. |
| Molecular mechanism: |
circ_0060937 functions as a ceRNA by sponging miR-195-5p to up-regulate HMGB3 expression, thereby promoting NSCLC progression and glycolysis. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); glycolysis (promotes); apoptosis (inhibits); cell cycle (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis; Actinomycin D / DRB Stability Assay; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; Western Blot; In Vivo Animal Model |
| Clinical significance: |
High circ_0060937 expression was associated with poor overall survival in NSCLC patients and the circ_0060937/miR-195-5p/HMGB3 axis might be a promising strategy for NSCLC therapy. |
| Description: |
circ_0060937 is up-regulated in NSCLC tissues and cells, and high expression is associated with poor overall survival. Functionally, circ_0060937 promotes NSCLC proliferation, migration, invasion, glycolysis and in vivo tumor growth while suppressing apoptosis. Mechanistically, cytoplasmic circ_0060937 sponges miR-195-5p to increase HMGB3 expression. |
| Confidence score: |
0.8571 |