| Expression pattern: |
UP |
| Associated gene: |
PVT1-104aa, c-Myc, MYC, GSK3beta, FBW7, PD-L1 |
| Associated microRNA: |
- |
| Biological function: |
circPVT1 is upregulated in CRC and functions as an oncogenic circRNA; its encoded product PVT1-104aa promotes CRC proliferation, colony formation, migration, metastasis, tumor growth, immune evasion, and response to combined anti-PD-L1 therapy. |
| Molecular mechanism: |
circPVT1 encodes PVT1-104aa via IRES-mediated translation; PVT1-104aa binds c-Myc, disrupts c-Myc interaction with GSK3beta and FBW7, suppresses ubiquitination-mediated c-Myc degradation, and increases PD-L1 expression through c-Myc-dependent transcriptional regulation. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); metastasis (promotes); invasion (promotes); ubiquitination (inhibits); immune regulation (promotes); T cell exhaustion (promotes); other pathway/process (promotes) |
| Detected method: |
Q
H
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; circRNA-seq; Actinomycin D / DRB Stability Assay; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; Western Blot; Co-IP; ChIP / ChIP-seq; Luciferase Reporter Assay; IHC (Immunohistochemistry); IF (Immunofluorescence); Flow Cytometry（Non-apoptosis/cycle）; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
PVT1-104aa upregulation is linked to deteriorated prognosis among individuals with CRC; elevated PVT1-104aa levels correlated significantly with adverse clinical outcomes, indicating its potential as a prognostic biomarker and therapeutic target. |
| Description: |
circPVT1 is a PVT1-derived circRNA that is upregulated in colorectal cancer tissues and cell lines. Its oncogenic effect is mediated mainly through translation of PVT1-104aa, which stabilizes c-Myc by inhibiting GSK3beta/FBW7-associated ubiquitination and thereby increases PD-L1 expression, promoting tumor growth, metastasis, immune evasion, and poorer prognosis. PVT1-104aa inhibition enhances anti-PD-L1 treatment efficacy in syngeneic CRC models. |
| Confidence score: |
0.8976 |