| Expression pattern: |
UP |
| Associated gene: |
TRPM2, GLI1, GLI2, NDUFB8, COX4 |
| Associated microRNA: |
miR-625-5p |
| Biological function: |
promotes proliferation, migration and invasion and inhibits apoptosis in prostate cancer cells; promotes tumor growth in vivo |
| Molecular mechanism: |
ceRNA mechanism: circEEF2 sponges miR-625-5p to upregulate TRPM2, activating Hedgehog signaling (GLI1/GLI2) and inducing mitochondrial stress (ROS, MMP changes, NDUFB8/COX4, gamma-H2AX). |
| Biological pathway or process: |
Hedgehog (promotes); mitochondrial function (other); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; Luciferase Reporter Assay; RNA Pull-Down; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; IF (Immunofluorescence); Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
circEEF2 is upregulated in prostate cancer and acts as an oncogenic circRNA. It sponges miR-625-5p to relieve repression of TRPM2, thereby activating Hedgehog signaling (GLI1/GLI2) and enhancing mitochondrial stress, promoting malignant phenotypes and xenograft growth. |
| Confidence score: |
0.7346 |