| Expression pattern: |
UP |
| Associated gene: |
RHOT1 |
| Associated microRNA: |
miR-1299 |
| Biological function: |
Promotes gastric cancer cell proliferation, invasion and migration; enhances EMT phenotype. |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-1299 to upregulate RHOT1, promoting EMT-related malignant behaviors in gastric cancer. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Sanger Sequencing; Back-Splice Junction PCR / divergent primers PCR; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Cell Cycle Assay; Western Blot; IHC (Immunohistochemistry); Luciferase Reporter Assay; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High expression is positively associated with histological grade, lymph node metastasis and TNM stages, and poor prognosis; potential diagnostic biomarker and prognosis assessment target for GC. |
| Description: |
In gastric cancer, hsa_circ_0005230 is up-regulated in tissues and cell lines and promotes proliferation, invasion and migration. Mechanistically, it mainly localizes to the cytoplasm and functions as a ceRNA by sponging miR-1299 to increase RHOT1 expression, thereby enhancing EMT-related malignant phenotypes and associating with poor prognosis. |
| Confidence score: |
0.8201 |