circRNA basic information
circBase ID: -
Name: hsa_circ_RPPH1
Synonym: hsa_circ_002144
Host Gene: RPPH1
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: exosome
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

BC tissue; paracancerous tissue; tumor tissue; adjacent normal tissue
Cell lines:

SK-BR-3; MDA-MB-231; MCF-10A; THP1
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: PKM, HK2, GLUT1, CD86, CD206, TNF-a, IL-1b, IL-10, TGF-b, IL-4, Arg I, IL-12, IFN-c
Associated microRNA: miR-326
Biological function: hsa_circ_002144 promotes breast cancer cell proliferation, migration, invasion, EMT, tumor growth and metastasis, inhibits apoptosis, enhances glycolysis, and promotes immune escape by inducing M2 macrophage polarization via exosomal transport.
Molecular mechanism: hsa_circ_002144 acts as a ceRNA sponge for miR-326 to upregulate PKM, thereby promoting glycolysis; it is also transported by exosomes from breast cancer cells into macrophages and affects macrophage glycolysis and M2 polarization.
Biological pathway or process:

glycolysis (promotes); immune regulation (promotes); macrophage polarization (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); apoptosis (inhibits); ceRNA regulation (promotes)
Detected method:
Q
H
Validation methods:

RNase R Treatment; RT-qPCR; FISH / smFISH; Clinical Sample Validation; Bioinformatics Analysis; Transfection; CCK8; Transwell Assay; Annexin V/PI Flow Cytometry; Flow Cytometry（Non-apoptosis/cycle）; ELISA; Western Blot; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); TUNEL; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); IF (Immunofluorescence)
Clinical significance:

hsa_circ_002144 could serve as a novel biomarker for breast cancer treatment and prognosis.
Description:

In this study, hsa_circ_002144 is identified as an upregulated breast cancer circRNA derived from RPPH1. It promotes malignant phenotypes including proliferation, migration, invasion, EMT, tumor growth and metastasis while inhibiting apoptosis. Mechanistically, hsa_circ_002144 sponges miR-326 to elevate PKM expression and promote glycolysis, and exosomal hsa_circ_002144 enters macrophages to induce M2 polarization and immune escape.
Confidence score:

0.8175
Other information
Title:

Hsa_Circ_002144 Promotes Glycolysis and Immune Escape of Breast Cancer Through miR-326/PKM Axis.

Journal: Cancer biotherapy & radiopharmaceuticals
Published: 2024
PubMed ID: 38963787
Study type:

combined biological and clinical study
Data availability: GSE101124; data sets available from the corresponding author on reasonable request
Code availability: -