| Expression pattern: |
UP |
| Associated gene: |
KPNA4, AGO2 |
| Associated microRNA: |
miR-516b-5p |
| Biological function: |
Promotes osteosarcoma cell viability and proliferation, enhances migration and invasion, and inhibits apoptosis; mediates the anti-tumor effects of Rg3. |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-516b-5p to increase KPNA4 expression (ceRNA mechanism), thereby promoting osteosarcoma malignant phenotypes; Rg3 suppresses this axis by reducing circ_0003074. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Transfection; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
circ_0003074 is up-regulated in osteosarcoma tissues and cells and promotes malignant phenotypes (proliferation, migration/invasion) while inhibiting apoptosis. Mechanistically, it sponges miR-516b-5p to de-repress KPNA4, and Rg3 exerts anti-osteosarcoma effects partly by reducing circ_0003074 and suppressing the circ_0003074/miR-516b-5p/KPNA4 axis. |
| Confidence score: |
0.8299 |