| Expression pattern: |
UP |
| Associated gene: |
ZNF652, SMAD5 |
| Associated microRNA: |
miR-3666 |
| Biological function: |
Promotes BC cell proliferation and migration, and decreases susceptibility of BC cells to NK cell-mediated cytotoxicity. |
| Molecular mechanism: |
ZNF652 transcriptionally induces circRHOT1; circRHOT1 acts as a miRNA sponge for miR-3666 to promote SMAD5 expression. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); immune regulation (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; EdU Staining; Transwell Assay; RNA Pull-Down; ChIP / ChIP-seq; Luciferase Reporter Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
circRHOT1 is up-regulated in bladder cancer and functions as an oncogenic circRNA by promoting proliferation and migration while reducing NK cell-mediated cytotoxicity. Mechanistically, ZNF652 transcriptionally induces circRHOT1, which sponges miR-3666 to upregulate SMAD5 (ZNF652/circRHOT1/miR-3666/SMAD5). |
| Confidence score: |
0.7435 |