| Expression pattern: |
DN |
| Associated gene: |
EPHB3, Ago2 |
| Associated microRNA: |
miR-637 |
| Biological function: |
suppresses GC cell proliferation, migration, invasion and anti-apoptosis; inhibits tumor growth and metastasis |
| Molecular mechanism: |
Exosomal circRELL1 functions as a ceRNA by sponging miR-637, thereby upregulating EPHB3 and modulating autophagy activation in gastric cancer. |
| Biological pathway or process: |
autophagy (inhibits); proliferation (inhibits); apoptosis (promotes); migration (inhibits); invasion (inhibits); metastasis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RT-qPCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; Clinical Sample Validation; Survival Analysis; ROC Analysis; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Annexin V/PI Flow Cytometry; TUNEL; Western Blot; IHC (Immunohistochemistry); IF (Immunofluorescence); In Vivo Animal Model; H&E Staining |
| Clinical significance: |
Decreased circRELL1 is associated with advanced TNM stage, lymph node metastasis and poor prognosis; plasma exosomal circRELL1 may serve as a circulating biomarker for GC diagnosis. |
| Description: |
circRELL1 (hsa_circ_0001400), derived from the RELL1 gene, is down-regulated in gastric cancer tissues and plasma exosomes and is associated with adverse clinicopathological features. Functionally, circRELL1 and exosomal circRELL1 suppress GC proliferation, migration/invasion, tumor growth and metastasis by acting as a ceRNA sponge for miR-637 to increase EPHB3 and modulate autophagy activity, supporting its potential as a circulating diagnostic/prognostic biomarker and therapeutic target. |
| Confidence score: |
0.8942 |