| Expression pattern: |
UP |
| Associated gene: |
CPEB4, Ago2 |
| Associated microRNA: |
miR-1270 |
| Biological function: |
Promotes CC cell proliferation, migration, invasion, tube formation and tumor growth, and inhibits apoptosis. |
| Molecular mechanism: |
Circ_0000069 acts as a ceRNA by sponging miR-1270 to upregulate CPEB4 expression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); angiogenesis (promotes); apoptosis (inhibits); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Bioinformatics Analysis; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; Tube Formation Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
Circ_0000069 expression was associated with clinical stage and HPV positivity in CC patients, and it might be a potential target for treatment of CC. |
| Description: |
Hsa_circ_0000069/circ_0000069 is upregulated in cervical cancer tissues and cell lines and correlates with clinical stage and HPV positivity. Functionally, it promotes malignant phenotypes including proliferation, migration, invasion, tube formation and xenograft tumor growth while suppressing apoptosis. Mechanistically, it acts as a cytoplasmic ceRNA that sponges miR-1270 to increase CPEB4 expression. |
| Confidence score: |
0.8572 |