| Expression pattern: |
UP |
| Associated gene: |
CCL5, UBAP2 |
| Associated microRNA: |
miR-139-3p |
| Biological function: |
promotes HCC cell proliferation and migration; promotes tumor growth; increases the ratio of M2/M1 macrophages by recruiting/polarizing M2 macrophages |
| Molecular mechanism: |
ceRNA mechanism: hsa_circ_0003410 sponges miR-139-3p to upregulate CCL5, leading to M2 macrophage recruitment/polarization and increased M2/M1 ratio |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); immune regulation (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; RNase R Treatment; Transfection; CCK8; EdU Staining; Transwell Assay; Wound Healing Assay; FISH / smFISH; RNA Pull-Down; Luciferase Reporter Assay; Western Blot; ELISA; Flow Cytometry（Non-apoptosis/cycle）; IF (Immunofluorescence); Clinical Sample Validation; Cohort Study; Survival Analysis; Bioinformatics Analysis; In Vivo Animal Model |
| Clinical significance: |
Expression of hsa_circ_0003410 was associated with tumor size; survival difference between high and low expression groups was not significant. |
| Description: |
hsa_circ_0003410 is up-regulated in HCC and promotes proliferation, migration, and in vivo tumor growth. It functions as a ceRNA by sponging miR-139-3p to upregulate CCL5, which enhances M2 macrophage recruitment/polarization and increases the M2/M1 macrophage ratio in the HCC microenvironment. |
| Confidence score: |
0.8428 |