| Expression pattern: |
UP |
| Associated gene: |
CCND1 |
| Associated microRNA: |
miR-106b, miR-106b-5p |
| Biological function: |
Promotes NSCLC cell proliferation and tumor growth. |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-106b-5p, thereby regulating CCND1 expression (ceRNA-like mechanism). |
| Biological pathway or process: |
proliferation (promotes); cell cycle (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
H
|
| Validation methods: |
RT-qPCR; ISH (In Situ Hybridization); Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; Western Blot; IF (Immunofluorescence); Colony Formation Assay; In Vivo Animal Model |
| Clinical significance: |
High circ_0000677 expression correlates with poor prognosis and worse overall survival in NSCLC patients. |
| Description: |
circ_0000677 is up-regulated in NSCLC tissues and predicts worse overall survival. It promotes NSCLC proliferation and in vivo tumor growth by sponging miR-106b-5p and de-repressing CCND1 (cyclin D1), forming a circ_0000677/miR-106b/CCND1 axis. |
| Confidence score: |
0.7357 |