| Expression pattern: |
DN |
| Associated gene: |
FLNA |
| Associated microRNA: |
miR-146a-5p |
| Biological function: |
Suppresses bladder cancer cell growth, proliferation, migration, motility and invasion; overexpression reduces tumor volume and weight in vivo. |
| Molecular mechanism: |
CircRNA ATF6 acts as a sponge for miR-146a-5p and suppresses BCa progression through the miR-146a-5p/FLNA axis. |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits); invasion (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Actinomycin D / DRB Stability Assay; RNase R Treatment; FISH / smFISH; Clinical Sample Validation; Bioinformatics Analysis; Transfection; CCK8; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); In Vivo Animal Model |
| Clinical significance: |
CircRNA ATF6 expression was correlated with lymphatic metastasis and invasion depth and may serve as a biomarker for BCa diagnosis and therapy. |
| Description: |
CircRNA ATF6 is down-regulated in bladder cancer tissues and cell lines and is associated with lymphatic metastasis and invasion depth. Functionally, circRNA ATF6 suppresses BCa cell proliferation, migration and invasion in vitro and reduces xenograft tumor growth in vivo. Mechanistically, it sponges miR-146a-5p to regulate FLNA expression, forming a circRNA ATF6/miR-146a-5p/FLNA axis with potential diagnostic and therapeutic relevance. |
| Confidence score: |
0.8351 |