| Expression pattern: |
DN |
| Associated gene: |
KAT7, HMGB1, H3K14ac, RNA polymerase II |
| Associated microRNA: |
- |
| Biological function: |
Relieves MI-induced myocardial ischemia/reperfusion injury and cardiomyocyte injury by suppressing autophagy, apoptosis, and inflammation; improves cardiac function in MI rat model. |
| Molecular mechanism: |
circFoxo3 represses KAT7, thereby reducing KAT7/H3K14ac/RNA pol II enrichment on the HMGB1 promoter and suppressing HMGB1 expression, leading to reduced autophagy and injury. |
| Biological pathway or process: |
autophagy (inhibits); apoptosis (inhibits); inflammation (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; Transfection; CCK8; Annexin V/PI Flow Cytometry; ELISA; Western Blot; IF (Immunofluorescence); ChIP / ChIP-seq; In Vivo Animal Model; H&E Staining; TUNEL |
| Clinical significance: |
- |
| Description: |
circFoxo3 is downregulated in MI/I/R models. circFoxo3 overexpression protects myocardium/cardiomyocytes by suppressing autophagy, apoptosis and inflammation, acting through a KAT7→HMGB1 transcriptional regulation module (reduced KAT7-associated H3K14ac and RNA pol II enrichment on the HMGB1 promoter). |
| Confidence score: |
0.6741 |