circRNA basic information
circBase ID: hsa_circ_0041103
Name: hsa_circ_TCF25
Synonym: hsa_circTCF25
Host Gene: TCF25
Genomic location(hg19): chr16:89962397-89967202:+
Genomic location(hg38): chr16:89895989-89900794:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0001056
MONDO name: gastric cancer
Disease details: gastric cancer
Disease DO ID:
 10534
Disease MeSH ID:
 -
Disease NCIt ID:
 C9331
Disease ICD11 ID:
 1397617262
Disease OMIM ID:
 613659
Species: Human
Species details: Homo sapiens
Tissue specimen:

gastric cancer tissues; paracancerous non-tumor tissues
Cell lines:

HGC-27; AGS; NCI-N87; MKN45; GES-1
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: IL6, FOS, ITGB3, BCL2L11, MAP2K1, HDAC2, PDGFRB, HNRNPA1, CARM1, BPTF, CD44, SOX2, OCT4
Associated microRNA: miR-149
Biological function: circTCF25 promotes gastric cancer cell proliferation, migration, invasion and cancer stem cell marker expression, and its knockdown represses cancer cell viability, migration, invasion and stemness-associated markers.
Molecular mechanism: circTCF25 appears to act as a miR-149 sponge and may regulate malignant activities in gastric cancer by targeting miR-149 and subsequently modulating downstream mRNA expression, including genes enriched in PI3K-AKT, MAPK and EGFR tyrosine kinase inhibitor resistance-related pathways.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); stemness (promotes); ceRNA regulation (promotes); PI3K/AKT (other); MAPK (other); EGFR (other); drug resistance (other)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Cohort Study; Survival Analysis; Transfection; CCK8; Transwell Assay; Luciferase Reporter Assay; Bioinformatics Analysis
Clinical significance:

circTCF25 was associated with lymph node metastasis, T stage, TNM stage and shorter overall survival; increased expression may serve as a biomarker to predict survival outcome in patients with gastric cancer.
Description:

In this study, circTCF25/hsa_circ_0041103 was up-regulated in human gastric cancer tissues and cell lines and high expression predicted shorter overall survival. Functional knockdown experiments indicated that circTCF25 promotes gastric cancer cell proliferation, migration, invasion and CSC marker expression. Mechanistically, the study supports a circTCF25-miR-149 regulatory network with predicted downstream mRNAs and cancer-related pathways such as PI3K-AKT and MAPK.
Confidence score:

0.7283
Other information
Title:

Hsa_circTCF25 Facilitates Oncogenesis in Gastric Cancer Cells by Modulating miR-149 Expression.

Journal: Anticancer research
Published: 2024
PubMed ID: 38537964
Study type:

combined biological and clinical study
Data availability: GSE78092; Gene Expression Omnibus repository
Code availability: -