| Expression pattern: |
UP |
| Associated gene: |
SKP2, AGO2 |
| Associated microRNA: |
miR-378a-3p |
| Biological function: |
promotes proliferation; promotes angiogenesis; accelerates G1/S transition; promotes xenograft growth |
| Molecular mechanism: |
Acts as a ceRNA sponge for miR-378a-3p to upregulate SKP2 expression, promoting HCC proliferation and angiogenesis. |
| Biological pathway or process: |
proliferation (promotes); angiogenesis (promotes); cell cycle (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RNA Pull-Down; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; EdU Staining; Cell Cycle Assay; Transwell Assay; Tube Formation Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circCRIM1 expression was associated with tumor size, TNM stage, Edmondson grade, and worse overall survival. |
| Description: |
CircCRIM1 (hsa_circ_0002346), derived from CRIM1, is upregulated in HCC tissues and cell lines and is mainly localized in the cytoplasm. It promotes HCC proliferation, G1/S cell-cycle progression and angiogenesis, and enhances xenograft growth by sponging miR-378a-3p to increase SKP2 expression (circCRIM1/miR-378a-3p/SKP2 axis). High circCRIM1 expression correlates with adverse clinicopathologic features and poorer overall survival. |
| Confidence score: |
0.8871 |