| Expression pattern: |
UP |
| Associated gene: |
TGIF2 |
| Associated microRNA: |
miR-214-3p |
| Biological function: |
Promotes tumor growth and malignant phenotypes (colony formation/proliferation, migration, invasion, cell cycle progression) and inhibits apoptosis; suppresses autophagy when highly expressed. |
| Molecular mechanism: |
Acts as a ceRNA/miRNA sponge for miR-214-3p to upregulate TGIF2; binding validated by luciferase reporter, RIP (Ago2), and RNA pulldown. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); cell cycle (promotes); autophagy (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Sanger Sequencing; Clinical Sample Validation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Transfection; Colony Formation Assay; Wound Healing Assay; Transwell Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circCPA4 expression is associated with tumor size, lymph node metastasis, TNM stage, and worse overall survival in lung cancer patients. |
| Description: |
circCPA4 is up-regulated in NSCLC and promotes lung cancer cell proliferation/colony formation, migration, invasion, and cell-cycle progression while inhibiting apoptosis. Mechanistically it functions as a ceRNA that sponges miR-214-3p to increase TGIF2 expression, enhancing tumor growth in a cell line-derived xenograft model; higher circCPA4 predicts worse survival. |
| Confidence score: |
0.8593 |