| Expression pattern: |
DN |
| Associated gene: |
- |
| Associated microRNA: |
miR-629 |
| Biological function: |
Inhibits migration, invasion, and epithelial-mesenchymal transition (EMT) in hepatocellular carcinoma cells. |
| Molecular mechanism: |
Acts as an miRNA sponge/target for miR-629; suppresses miR-629 expression, and miR-629 mimics reverse circSMAD2-mediated inhibition of migration, invasion and EMT. |
| Biological pathway or process: |
EMT (inhibits); migration (inhibits); invasion (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; Transwell Assay; Western Blot; CCK8; Annexin V/PI Flow Cytometry |
| Clinical significance: |
circSMAD2 expression was associated with the differentiation degree of the HCC tissues (lower in poorly differentiated tissues). |
| Description: |
circSMAD2 is down-regulated in hepatocellular carcinoma tissues and HCC cell lines. Functionally, circSMAD2 suppresses migration, invasion and EMT in HCC cells by binding/negatively regulating miR-629, and miR-629 overexpression reverses these inhibitory effects. |
| Confidence score: |
0.7581 |