| Expression pattern: |
UP |
| Associated gene: |
p53, Bax, Bcl-2, E-cadherin, N-cadherin, Vimentin |
| Associated microRNA: |
miR-525-3p |
| Biological function: |
Promotes CRC cell proliferation, migration/invasion (metastasis-related behaviors) and inhibits apoptosis; knockdown suppresses growth and metastasis and increases apoptosis. |
| Molecular mechanism: |
Acts as a miRNA sponge for miR-525-3p; affects p53 signaling and EMT markers (E-cadherin, N-cadherin, vimentin), contributing to proliferation/apoptosis/metastasis phenotypes. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); metastasis (promotes); EMT (promotes); p53 signaling (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot |
| Clinical significance: |
Patients with CRC and circRNA-0074027 overexpression were more likely to have low tumor differentiation, lymph node metastasis and advanced TMN stage. |
| Description: |
circRNA-0074027 is up-regulated in colorectal cancer tissues/cell lines and functions as a tumor promoter. Its knockdown inhibits proliferation and migration/invasion and increases apoptosis, at least partly by sponging miR-525-3p and modulating p53 signaling and EMT-related markers. |
| Confidence score: |
0.6536 |