| Expression pattern: |
UP |
| Associated gene: |
NUCKS1 |
| Associated microRNA: |
miR-433-3p |
| Biological function: |
promotes tumor growth, proliferation, migration, invasion, and the Warburg effect in NSCLC |
| Molecular mechanism: |
ceRNA mechanism: circ_0008037 sponges miR-433-3p to upregulate NUCKS1 |
| Biological pathway or process: |
glycolysis (promotes); proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis; RNA Pull-Down; Luciferase Reporter Assay; Transfection; MTT; Colony Formation Assay; Transwell Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
high circ_0008037 expression predicted a worse prognosis in a cohort of 56 NSCLC patients; high circ_0008037 expression was related to tumor size, TNM stage, and lymphatic metastasis |
| Description: |
In NSCLC, circ_0008037 is up-regulated and mainly localized in the cytoplasm. It promotes tumor growth, proliferation, migration/invasion and glycolytic Warburg effect by acting as a ceRNA to sponge miR-433-3p and thereby increase NUCKS1 expression; high expression is associated with worse patient prognosis. |
| Confidence score: |
0.8593 |