circRNA basic information
circBase ID: hsa_circ_0007624
Name: hsa_circ_BCAR3
Synonym: circ_0007624
Host Gene: BCAR3
Genomic location(hg19): chr1:94047857-94057950:-
Genomic location(hg38): chr1:93582300-93592393:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005580
MONDO name: esophageal squamous cell carcinoma
Disease details: esophageal squamous cell carcinoma
Disease DO ID:
 3748
Disease MeSH ID:
 C562729; D000077277
Disease NCIt ID:
 C4024
Disease ICD11 ID:
 1417891145
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

ESCC tumor tissues; adjacent normal esophageal tissues
Cell lines:

TE-1; EC9706; Het-1A
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: CPEB3, EGFR, PI3K, AKT
Associated microRNA: miR-224-5p
Biological function: Suppresses ESCC cell proliferation, migration, invasion, metastasis and EMT, promotes apoptosis, induces G0/G1 cell cycle arrest, and slows xenograft tumor growth.
Molecular mechanism: Acts as a ceRNA sponge for miR-224-5p, relieves miR-224-5p-mediated repression of CPEB3, and inactivates EGFR/PI3K/AKT signaling.
Biological pathway or process:

EGFR (inhibits); PI3K/AKT (inhibits); proliferation (inhibits); migration (inhibits); invasion (inhibits); metastasis (inhibits); EMT (inhibits); apoptosis (promotes); cell cycle (inhibits); ceRNA regulation (other)
Detected method:
Q
M
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Microarray; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis
Clinical significance:

Low circ_0007624 expression was associated with poor prognosis, advanced TNM stage and lymph node metastasis in ESCC patients.
Description:

circ_0007624 is down-regulated in ESCC and low expression predicts worse survival. It functions as a tumor suppressor by sponging miR-224-5p, increasing CPEB3 expression, and inactivating EGFR/PI3K/AKT signaling, thereby inhibiting proliferation, metastasis and EMT while promoting apoptosis.
Confidence score:

0.8921
Other information
Title:

Circ_0007624 suppresses the development of esophageal squamous cell carcinoma via targeting miR-224-5p/CPEB3 to inactivate the EGFR/PI3K/AKT signaling.

Journal: Cellular signalling
Published: 2022
PubMed ID: 35998761
Study type:

combined biological and clinical study
Data availability: GSE112496; GSE150476; GSE114110; GSE135918; GSE38129; GSE20347; GSE17351; GSE44021; Supplementary data at https://doi.org/10.1016/j.cellsig.2022.110448
Code availability: -