| Expression pattern: |
UP |
| Associated gene: |
TMTC3, AGO2 |
| Associated microRNA: |
miR-1305 |
| Biological function: |
Promotes HCC cell proliferation, invasion, migration, tumor growth and lung metastasis; knockdown inhibits these malignant phenotypes. |
| Molecular mechanism: |
circ_0008934 acts as a ceRNA sponge for miR-1305, thereby releasing/facilitating TMTC3 expression and promoting HCC growth and metastasis. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes); other pathway/process (other) |
| Detected method: |
Q
S
|
| Validation methods: |
Sanger Sequencing; RNase R Treatment; RT-qPCR; RNA-seq; FISH / smFISH; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circ_0008934 expression is associated with higher serum AFP, larger tumor diameter, microvascular invasion, poorer overall survival, and is an independent prognostic factor for poor OS in HCC patients. |
| Description: |
circ_0008934 is up-regulated in HCC tissues and cell lines and high expression predicts poor overall survival and aggressive clinicopathological features. Functionally, circ_0008934 promotes HCC proliferation, invasion, migration, tumor growth and lung metastasis. Mechanistically, it acts as a miR-1305 sponge to increase TMTC3 expression, forming a circ_0008934/miR-1305/TMTC3 regulatory axis. |
| Confidence score: |
0.8709 |