circRNA basic information
circBase ID: -
Name: hsa_circ_ZCCHC6
Synonym: circZCCHC6
Host Gene: ZCCHC6
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

serum; cancerous tissues; adjacent normal tissues; tumor tissues
Cell lines:

BEAS-2B; A549; H522; H1975; HCC827; PC-9
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: LPCAT1
Associated microRNA: miR-433-3p
Biological function: Promotes NSCLC cell viability/proliferation, cell cycle progression, migration, invasion, EMT, tumor growth, and metastasis; circZCCHC6 knockdown suppresses these malignant phenotypes.
Molecular mechanism: circZCCHC6 acts as a ceRNA/sponge for miR-433-3p, thereby upregulating LPCAT1 expression and promoting NSCLC progression and EMT.
Biological pathway or process:

proliferation (promotes); cell cycle (promotes); migration (promotes); invasion (promotes); EMT (promotes); metastasis (promotes); ceRNA regulation (other)
Detected method:
Q
B
Validation methods:

RNase R Treatment; RT-qPCR; Northern Blot; Clinical Sample Validation; Transfection; MTT; Cell Cycle Assay; Wound Healing Assay; Transwell Assay; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Western Blot; In Vivo Animal Model; ROC Analysis; Bioinformatics Analysis
Clinical significance:

Plasma/serum circZCCHC6 may serve as a diagnostic biomarker for NSCLC; ROC AUC = 0.7895.
Description:

circZCCHC6 is up-regulated in NSCLC serum, tissues, and cell lines and shows diagnostic potential. Functionally, it promotes NSCLC cell proliferation, cell cycle progression, migration, invasion, EMT, and xenograft tumor growth. Mechanistically, circZCCHC6 sponges miR-433-3p to positively regulate LPCAT1 expression, forming a circZCCHC6/miR-433-3p/LPCAT1 axis.
Confidence score:

0.8324
Other information
Title:

Circular RNA circZCCHC6 contributes to tumorigenesis by regulating LPCAT1 via miR-433-3p in non-small cell lung cancer.

Journal: Clinical and experimental medicine
Published: 2022
PubMed ID: 35089454
Study type:

combined biological and clinical study
Data availability: -
Code availability: -