| Expression pattern: |
UP |
| Associated gene: |
YWHAB |
| Associated microRNA: |
miR-144-5p |
| Biological function: |
promotes proliferation, colony formation, migration and invasion/metastasis; promotes tumor growth in vivo |
| Molecular mechanism: |
ceRNA sponge mechanism: circ_0006282 sponges miR-144-5p to upregulate YWHAB |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; In Vivo Animal Model; Clinical Sample Validation |
| Clinical significance: |
- |
| Description: |
circ_0006282 is upregulated in gastric cancer tissues and cell lines, mainly localized in the cytoplasm, and promotes GC cell proliferation and metastatic phenotypes. Mechanistically, it acts as a ceRNA by sponging miR-144-5p to increase YWHAB expression; knockdown suppresses tumor growth in a nude mouse xenograft model. |
| Confidence score: |
0.7827 |