| Expression pattern: |
UP |
| Associated gene: |
AKT3, MMP2, MMP9 |
| Associated microRNA: |
miR-296-3p |
| Biological function: |
Promotes NSCLC cell growth and metastasis (increases viability, colony formation, migration, invasion; inhibits apoptosis). |
| Molecular mechanism: |
Acts as a ceRNA by sponging miR-296-3p to relieve repression of AKT3, leading to increased AKT3 expression. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis; Luciferase Reporter Assay; RNA Pull-Down; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot |
| Clinical significance: |
High circWHSC1 expression is associated with decreased overall survival and is an independent indicator of poor prognosis in NSCLC patients. |
| Description: |
circWHSC1 is up-regulated in NSCLC tissues/cells and predicts poorer overall survival. It promotes proliferation and metastatic phenotypes and inhibits apoptosis by sponging miR-296-3p to up-regulate AKT3. |
| Confidence score: |
0.7548 |