| Expression pattern: |
UP |
| Associated gene: |
PRKAA1 |
| Associated microRNA: |
miR-873-5p |
| Biological function: |
promotes proliferation, migration, invasion, and glycolysis of gastric cancer cells under hypoxia; promotes tumor growth and glycolysis in vivo |
| Molecular mechanism: |
ceRNA sponge mechanism: circC6orf132 sponges miR-873-5p to positively regulate PRKAA1 expression |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); glycolysis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Transfection; Colony Formation Assay; Transwell Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation |
| Clinical significance: |
- |
| Description: |
circC6orf132 is up-regulated in gastric cancer tissues/cells and further induced by hypoxia. It mainly localizes to the cytoplasm and promotes hypoxia-associated malignant phenotypes (proliferation, migration, invasion) and glycolysis by sponging miR-873-5p, thereby increasing PRKAA1 expression; knockdown suppresses xenograft tumor growth and glycolysis. |
| Confidence score: |
0.8025 |